IHC-P analysis of rat skeletal muscle tissue using GTX25619 MUSK antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave
Permeabilization : TBS/0.025% Triton
Dilution : 1:20

WB analysis of 3ng of muscle-specific kinase antigen using GTX25619 MUSK antibody.
Dilution : 1 μg/ml

WB analysis of 30 ug of rat skeletal muscle tissue lysate using GTX25619 MUSK antibody.
Dilution : 1 μg/ml

ICC/IF analysis of C2C12 cells using GTX25619 MUSK antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:20 in blocking buffer for at least 1 hour at room temperature

IHC-P analysis of mouse skeletal muscle tissue using GTX25619 MUSK antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave
Permeabilization : TBS/0.025% Triton
Dilution : 1:20

ICC/IF analysis of L6 cells using GTX25619 MUSK antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:20 in blocking buffer for at least 1 hour at room temperature

IHC-P analysis of human skeletal muscle tissue using GTX25619 MUSK antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave
Permeabilization : TBS/0.025% Triton
Dilution : 1:20