APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
human MX1-transfected 293T cells
Product Note
This antibody is specific for human MX1 protein, and it does not cross react with human MX2 protein.
Predict Reactivity
Mouse, Sheep, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MX1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888140
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MX dynamin like GTPase 1 , IFI-78K , IFI78 , MX , MxA
Cellular Localization
Cytoplasm
Background
In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738](/templates/default/js/lightbox/images/loading.gif "Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738")
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GTX629738 WB Image
MX1 antibody [GT4812] detects MX1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human MX1-transfected 293T cells
7.5 % SDS-PAGE
MX1 antibody [GT4812] (GTX629738) dilution: 1:10000
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GTX629738 ICC/IF Image
MX1 antibody [GT4812] detects MX1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: MX1 protein stained by MX1 antibody [GT4812] (GTX629738) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX629738 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [GT4812] (GTX629738) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX629738 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [GT4812] (GTX629738) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for MX1 antibody [GT4812] (GTX629738). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MX1 antibody [GT4812] (GTX629738). Be the first to share your experience with this product.
![Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738](/upload/website/prouct_img/small/GTX629738/GTX629738_41421_WB_B_w_23061202_329.webp "Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738")
![Anti-MX1 antibody [GT4812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629738](/upload/website/prouct_img/small/GTX629738/GTX629738_41421_20151111_IFA_w_23061202_514.webp "Anti-MX1 antibody [GT4812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629738")
![Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738](/upload/website/prouct_img/small/GTX629738/GTX629738_45154_20230901_WB_TPM_watermark_23091319_413.webp "Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738")
![Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738](/upload/website/prouct_img/small/GTX629738/GTX629738_45154_20230915_WB_multiple_B_23091901_600.webp "Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738")
![Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738](/upload/website/prouct_img/normal/GTX629738/GTX629738_41421_WB_B_w_23061202_329.webp "Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738")
![Anti-MX1 antibody [GT4812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629738](/upload/website/prouct_img/normal/GTX629738/GTX629738_41421_20151111_IFA_w_23061202_514.webp "Anti-MX1 antibody [GT4812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629738")
![Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738](/upload/website/prouct_img/normal/GTX629738/GTX629738_45154_20230901_WB_TPM_watermark_23091319_413.webp "Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738")
![Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738](/upload/website/prouct_img/normal/GTX629738/GTX629738_45154_20230915_WB_multiple_B_23091901_600.webp "Anti-MX1 antibody [GT4812] used in Western Blot (WB). GTX629738")