MX1 antibody [N2C2], Internal

Cat. No. GTX110256

Reference 19

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Rabbit, Bovine, Monkey
Package	100 μl ($399), 25 μl ($169)

See all MX1 products

View product citations for antibody GTX110256 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent

Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

human MX1-transfected 293T

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950963

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MX dynamin like GTPase 1 , IFI-78K , IFI78 , MX , MxA

Cellular Localization

Cytoplasm

Background

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Gene ID: 4599 MX1
UniProt: P20591 MX1

Research Area

DATA IMAGES

GTX110256 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody (GTX110256) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110256 ICC/IF Image

MX1 antibody [N2C2], Internal detects MX1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MX1 protein stained by MX1 antibody [N2C2], Internal (GTX110256) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX110256 IHC-P Image

MX1 antibody [N2C2], Internal detects MX1 protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
MX1 antibody [N2C2], Internal (GTX110256) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110256 WB Image

MX1 antibody [N2C2], Internal detects MX1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human MX1-transfected 293T cells.
7.5% SDS-PAGE
MX1 antibody [N2C2], Internal (GTX110256) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110256 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using MX1(GTX110256) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min