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MX1 antibody

Orthogonal Overexpression
Anti-MX1 antibody used in Western Blot (WB). GTX111153
Anti-MX1 antibody used in Western Blot (WB). GTX111153
Anti-MX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111153
Anti-MX1 antibody used in Western Blot (WB). GTX111153

Cat. No. GTX111153

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

human MX1-transfected 293T , NCI-H929

Predict Reactivity

Mouse, Sheep, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950964

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MX dynamin like GTPase 1 , IFI-78K , IFI78 , MX , MxA

Cellular Localization

Cytoplasm

Background

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MX1 antibody used in Western Blot (WB). GTX111153

GTX111153 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody (GTX111153) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MX1 antibody used in Western Blot (WB). GTX111153

GTX111153 WB Image

Overexpression

MX1 antibody detects MX1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human MX1-transfected 293T cells
7.5% SDS-PAGE
MX1 antibody (GTX111153) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111153

GTX111153 IHC-P Image

MX1 Antobody detects MX1 protein at cytosol on MDA-MB-468 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-468 xenograft.
MX1 Antobody (GTX111153) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MX1 antibody used in Western Blot (WB). GTX111153

GTX111153 WB Image

Orthogonal

The data was published in the journal mBio in 2017. PMID: 28465426

REFERENCE

Application Reference

Botto S et al. mBio 2019; 10 (1) Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome.
Pryke KM et al. MBio 2017; 8 (3) A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169