APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.87mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MYBPC2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
MGC163408 antibody, MYBPC antibody, MYBPCF antibody, MYBPC2 antibody, "C-protein, skeletal muscle fast isoform antibody", fast MyBP-C antibody, fast-type muscle myosin-binding-protein C antibody, "myosin-binding protein C, fast-type antibody", "myosin binding protein C, fast type antibody"
Background
Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
Database
Research Area
DATA IMAGES
|
GTX105777 WB Image
Sample (30 ug of whole cell lysate)
A: Hela S3
7.5 % SDS PAGE
GTX105777 diluted at 1:500
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GTX105777 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MYBPC2(GTX105777) antibody at 1:200 dilution.
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