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MYF5 antibody

Anti-MYF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX87110
Anti-MYF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87110
Anti-MYF5 antibody used in Western Blot (WB). GTX87110

Cat. No. GTX87110

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Monkey
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MYF5 (21-70).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10724548

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MYF5 , bHLHc2 , myogenic factor 5

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-MYF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX87110

GTX87110 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX87110 MYF5 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MYF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87110

GTX87110 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX87110 MYF5 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MYF5 antibody used in Western Blot (WB). GTX87110

GTX87110 WB Image

WB analysis of COS7 cell lysates using GTX87110 MYF5 antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

REVIEW

MYF5 antibody Cat. No. GTX87110

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 28467493
Date : Anonymous submitted on 08-Jan-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : C2C12 cells
Amount used : 40μg
Blocking : 1% BSA,10%HS,0.075% Tween 20 in Tris-Cl pH7.4, 25°C, 1Hr
Primary Antibody : 1:250, 4°C, 16Hr
Lane Description : As MYF5 is a key transcription factor in myogenic specification and initial differentiation, WB was performed on C2C12 mouse myoblasts cultured in proliferation medium (PM) and then shifted to differentiation medium (DM) to allow myotubes formation.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399