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MYH10 antibody [GT246]

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634160
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in IHC (Frozen sections) (IHC-Fr). GTX634160
Anti-MYH10 antibody [GT246] used in IHC (Paraffin sections) (IHC-P). GTX634160

Cat No. GTX634160

Host Mouse
Clonality Monoclonal
Clone Name GT246
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

229 kDa. ( Note )

Positive Control

HeLa , MYH10-transfected 293T , NIH-3T3 , mouse brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myosin Heavy Chain 10,Nmmhc-Iib,Nmmhcb,Myh10

Background

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MYH10 antibody [GT246] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634160

GTX634160 ICC/IF Image

MYH10 antibody [GT246] detects MYH10 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MYH10 protein stained by MYH10 antibody [GT246] (GTX634160) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000.

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MYH10 antibody [GT246] used in IHC (Frozen sections) (IHC-Fr). GTX634160

GTX634160 IHC-Fr Image

MYH10 antibody [GT246] detects MYH10 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MYH10 stained by MYH10 antibody [GT246] (GTX634160) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-MYH10 antibody [GT246] used in IHC (Paraffin sections) (IHC-P). GTX634160

GTX634160 IHC-P Image

MYH10 antibody [GT246] detects MYH10 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MYH10 antibody [GT246] (GTX634160) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169