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MYH10 antibody [GT246]

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in IHC (Frozen sections) (IHC-Fr). GTX634160
Anti-MYH10 antibody [GT246] used in IHC (Paraffin sections) (IHC-P). GTX634160
Anti-MYH10 antibody [GT246] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634160
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

Cat No. GTX634160

Host

Mouse

Clonality

Monoclonal

Clone Name

GT246

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

229 kDa. ( Note )

Positive Control

HeLa , MYH10-transfected 293T , NIH-3T3 , mouse brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888411

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin heavy chain 10 , NMMHC-IIB , NMMHCB

Background

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MYH10 antibody [GT246] used in IHC (Frozen sections) (IHC-Fr). GTX634160

GTX634160 IHC-Fr Image

MYH10 antibody [GT246] detects MYH10 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MYH10 stained by MYH10 antibody [GT246] (GTX634160) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-MYH10 antibody [GT246] used in IHC (Paraffin sections) (IHC-P). GTX634160

GTX634160 IHC-P Image

MYH10 antibody [GT246] detects MYH10 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MYH10 antibody [GT246] (GTX634160) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MYH10 antibody [GT246] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634160

GTX634160 ICC/IF Image

MYH10 antibody [GT246] detects MYH10 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MYH10 protein stained by MYH10 antibody [GT246] (GTX634160) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000.

REFERENCE

There are currently no references for MYH10 antibody [GT246] (GTX634160). Be the first to share your publications with this product.

REVIEW

MYH10 antibody [GT246] Cat No. GTX634160

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 19-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : A10 cells
Amount used : 60μg
Blocking : 5% non-fat milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169