APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , MYH10-transfected 293T , NIH-3T3 , mouse brain
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888401
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
myosin heavy chain 10 , NMMHC-IIB , NMMHCB
Background
This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Database
Research Area
DATA IMAGES
![Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139](/templates/default/js/lightbox/images/loading.gif "Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139")
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GTX634139 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT324] (GTX634139) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634139 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT324] (GTX634139) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634139 IHC-P Image
MYH10 antibody [GT324] detects MYH10 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MYH10 antibody [GT324] (GTX634139) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634139 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT324] (GTX634139) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634139 IHC-P Image
MYH10 antibody [GT324] detects MYH10 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.MYH10 stained by MYH10 antibody [GT324] (GTX634139) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634139 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT324] (GTX634139) diluted at 1:5000.
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GTX634139 IHC-Fr Image
MYH10 antibody [GT324] detects MYH10 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MYH10 stained by MYH10 antibody [GT324] (GTX634139) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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REFERENCE
There are currently no references for MYH10 antibody [GT324] (GTX634139). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MYH10 antibody [GT324] (GTX634139). Be the first to share your experience with this product.
![Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139](/upload/website/prouct_img/small/GTX634139/GTX634139_42786_20170323_WB_B_w_23061202_805.webp "Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139")
![Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139](/upload/website/prouct_img/small/GTX634139/GTX634139_42786_20160629_WB_M_w_23061202_286.webp "Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139")
![Anti-MYH10 antibody [GT324] used in IHC (Paraffin sections) (IHC-P). GTX634139](/upload/website/prouct_img/small/GTX634139/GTX634139_42786_20170711_IHC-P_R_1_w_23061202_791.webp "Anti-MYH10 antibody [GT324] used in IHC (Paraffin sections) (IHC-P). GTX634139")
![Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139](/upload/website/prouct_img/small/GTX634139/GTX634139_42786_20160629_WB_M_brain_w_23061202_603.webp "Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139")
![Anti-MYH10 antibody [GT324] used in IHC (Paraffin sections) (IHC-P). GTX634139](/upload/website/prouct_img/small/GTX634139/GTX634139_42786_20190719_IHC-P_R_1_w_23061202_199.webp "Anti-MYH10 antibody [GT324] used in IHC (Paraffin sections) (IHC-P). GTX634139")
![Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139](/upload/website/prouct_img/small/GTX634139/GTX634139_42786_20170309_WB_w_23061202_307.webp "Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139")
![Anti-MYH10 antibody [GT324] used in IHC (Frozen sections) (IHC-Fr). GTX634139](/upload/website/prouct_img/small/GTX634139/GTX634139_42786_20170831_IHC-Fr_M_w_23061202_504.webp "Anti-MYH10 antibody [GT324] used in IHC (Frozen sections) (IHC-Fr). GTX634139")
![Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139](/upload/website/prouct_img/normal/GTX634139/GTX634139_42786_20170323_WB_B_w_23061202_805.webp "Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139")
![Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139](/upload/website/prouct_img/normal/GTX634139/GTX634139_42786_20160629_WB_M_w_23061202_286.webp "Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139")
![Anti-MYH10 antibody [GT324] used in IHC (Paraffin sections) (IHC-P). GTX634139](/upload/website/prouct_img/normal/GTX634139/GTX634139_42786_20170711_IHC-P_R_1_w_23061202_791.webp "Anti-MYH10 antibody [GT324] used in IHC (Paraffin sections) (IHC-P). GTX634139")
![Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139](/upload/website/prouct_img/normal/GTX634139/GTX634139_42786_20160629_WB_M_brain_w_23061202_603.webp "Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139")
![Anti-MYH10 antibody [GT324] used in IHC (Paraffin sections) (IHC-P). GTX634139](/upload/website/prouct_img/normal/GTX634139/GTX634139_42786_20190719_IHC-P_R_1_w_23061202_199.webp "Anti-MYH10 antibody [GT324] used in IHC (Paraffin sections) (IHC-P). GTX634139")
![Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139](/upload/website/prouct_img/normal/GTX634139/GTX634139_42786_20170309_WB_w_23061202_307.webp "Anti-MYH10 antibody [GT324] used in Western Blot (WB). GTX634139")
![Anti-MYH10 antibody [GT324] used in IHC (Frozen sections) (IHC-Fr). GTX634139](/upload/website/prouct_img/normal/GTX634139/GTX634139_42786_20170831_IHC-Fr_M_w_23061202_504.webp "Anti-MYH10 antibody [GT324] used in IHC (Frozen sections) (IHC-Fr). GTX634139")