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MYH10 antibody

Cat No. GTX11090

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Rat, Dog
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200
Not tested in other applications.

Calculated MW

229 kDa. ( Note )

Positive Control

MDCK, Jurkat, NRK

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acid residues 1965-1976 of the heavy chain of human myosin IIB (nonmuscle) conjugated to KLH. This sequence is identical in rabbit and differs by one and two amino acids from the respective bovine and rat sequences.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin heavy chain 10 , MCH-B , NMMHC-B , SMemb

Background

Myosins belong to a superfamily of actin based motor proteins comprising to date at least 15 classes. There are two main groups of myosins: the conventional (class II) and the unconventional myosins. Myosin IIB (nonmuscle) is a relatively abundant, widespread two headed myosin composed of an N-terminal motor domain, a light chain binding neck region, a coiled coil region, and a nonhelical C-terminal domain. It forms a heterohexamer composed of a pair of heavy chains and two pairs of light chains. The vertebrate myosin II class members include muscle (sarcomeric and smooth) and nonmuscle (cytoplasmic) myosins. The non muscle myosin II class contains two isoforms: IIA and IIB, which exhibit 85% and 72% amino acid identity in the motor domain and the rod, respectively. Varying ratios of the two isoforms are expressed in different cells and tissues. Some cells express only a single isotype: e.g. myosin IIA in human platelets, rat basophilic leukemia cells, and chicken intestinal epithelium and myosin IIB in embryonic cardiac myocytes and COS-7 cells. The two isoforms vary in their intracellular localization, enzymatic activities, and proposed functions. Three additional isoforms of IIB have been described in neurons. Another isoform, myosin IIC, seems to constitute a distinct third isoform of the nonmuscle myosins. Nonmuscle myosin II is involved in cell motility and adhesion, cytokinesis, vesicular transport, intracellular force generation, and in morphogenesis during development. Its activity is regulated by light chain and possibly heavy chain phosphorylation and by association with proteins such as Mts1. Lethal defects in the development of the heart and hydrocephalus of the brain have been described in NMHCB homozygous knockout mice.

Database

Research Area

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349