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MYH10 antibody

Overexpression
Anti-MYH10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133378
Anti-MYH10 antibody used in Western Blot (WB). GTX133378
Anti-MYH10 antibody used in Western Blot (WB). GTX133378

Cat. No. GTX133378

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MYH10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

229 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , 293T , MYH10-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886964

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin heavy chain 10 , NMMHC-IIB , NMMHCB

Background

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-MYH10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133378

GTX133378 ICC/IF Image

MYH10 antibody detects MYH10 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MYH10 protein stained by MYH10 antibody (GTX133378) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-MYH10 antibody used in Western Blot (WB). GTX133378

GTX133378 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody (GTX133378) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MYH10 antibody used in Western Blot (WB). GTX133378

GTX133378 WB Image

Overexpression

Non-transfected (–) and transfected (+) MYH10-transfected 293T (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody (GTX133378) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

SH Lu et al. Stem Cell Reports 2023; S2213-6711 (23) : 00184-4 Cardiac myofibrillogenesis is spatiotemporally modulated by the molecular chaperone UNC45B
Application : WB, ICC/IF
Reactivity : Human
Ruobo Zhou et al. Nat Commun 2022; 13 (1) : 3196 Proteomic and functional analyses of the periodic membrane skeleton in neurons.
Lu H.A. et al. Circ Res. 2022; 130 (1) : 112-129 Alternative Splicing Mediated by RNA-Binding Protein RBM24 Facilitates Cardiac Myofibrillogenesis in a Differentiation Stage-Specific Manner.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169