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MYH10 antibody

Anti-MYH10 antibody used in Western Blot (WB). GTX133378
Anti-MYH10 antibody used in Western Blot (WB). GTX133378
Anti-MYH10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133378

Cat No. GTX133378

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

229 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , MYH10-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886964

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin heavy chain 10 , NMMHC-IIB , NMMHCB

Background

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-MYH10 antibody used in Western Blot (WB). GTX133378

GTX133378 WB Image

Non-transfected (–) and transfected (+) MYH10-transfected 293T (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody (GTX133378) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MYH10 antibody used in Western Blot (WB). GTX133378

GTX133378 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody (GTX133378) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MYH10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133378

GTX133378 ICC/IF Image

MYH10 antibody detects MYH10 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MYH10 protein stained by MYH10 antibody (GTX133378) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169