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MYH3 antibody

Overexpression
Anti-MYH3 antibody used in IHC (Paraffin sections) (IHC-P). GTX32147
Anti-MYH3 antibody used in Western Blot (WB). GTX32147
Anti-MYH3 antibody used in Western Blot (WB). GTX32147

Cat. No. GTX32147

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all MYH3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

224 kDa. ( Note )

Positive Control

Fetal Human Skeletal Muscle Lysate

Product Note

MYH3 antibody is predicted to not cross-react with other members of the myosin heavy chain family.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MYH3 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH3.The immunogen is located within amino acids 30 - 80 of MYH3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887639

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin heavy chain 3 , DA2A , DA2B , DA8 , HEMHC , MYHC-EMB , MYHSE1 , SMHCE

Background

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MYH3 antibody used in IHC (Paraffin sections) (IHC-P). GTX32147

GTX32147 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX32147 MYH3 antibody.
Working concentration : 5 μg/ml

Anti-MYH3 antibody used in Western Blot (WB). GTX32147

GTX32147 WB Image

WB analysis of fetal human skeletal muscle lysate using GTX32147 MYH3 antibody.
Working concentration : 1 μg/ml

Anti-MYH3 antibody used in Western Blot (WB). GTX32147

GTX32147 WB Image

Overexpression

The data was published in the journal Sci Rep in 2017. PMID: 28205584

REFERENCE

Application Reference

Vaiman A et al. Genet Sel Evol 2022; 54 (1) : 71 Mutation of the MYH3 gene causes recessive cleft palate in Limousine cattle.
Zieba J et al. Sci Rep 2017; 7 : 41803 A postnatal role for embryonic myosin revealed by MYH3 mutations that alter TGFβ signaling and cause autosomal dominant spondylocarpotarsal synostosis.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399