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MYH3 antibody

Overexpression
Anti-MYH3 antibody used in Western Blot (WB). GTX32147
Anti-MYH3 antibody used in IHC (Paraffin sections) (IHC-P). GTX32147

Cat. No. GTX32147

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all MYH3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

224 kDa. ( Note )

Positive Control

Fetal Human Skeletal Muscle Lysate

Product Note

MYH3 antibody is predicted to not cross-react with other members of the myosin heavy chain family.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MYH3 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH3.The immunogen is located within amino acids 30 - 80 of MYH3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887639

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin heavy chain 3 , DA2A , DA2B , DA8 , HEMHC , MYHC-EMB , MYHSE1 , SMHCE

Background

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MYH3 antibody used in Western Blot (WB). GTX32147

GTX32147 WB Image

WB analysis of fetal human skeletal muscle lysate using GTX32147 MYH3 antibody.
Working concentration : 1 μg/ml

Anti-MYH3 antibody used in IHC (Paraffin sections) (IHC-P). GTX32147

GTX32147 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX32147 MYH3 antibody.
Working concentration : 5 μg/ml

REFERENCE

Application Reference

Zieba J et al. Sci Rep 2017; 7 : 41803 A postnatal role for embryonic myosin revealed by MYH3 mutations that alter TGFβ signaling and cause autosomal dominant spondylocarpotarsal synostosis.
Application : IHC-P
Reactivity : Mouse
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REVIEW

MYH3 antibody Cat. No. GTX32147

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-MYH3 antibody used in Western Blot (WB). GTX32147
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28205584
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399