GeneTex
United States (US)

MYH7 antibody [N1], N-term

GTX100713_40058_20160505_WB_M_muscle_19011506_580.jpg

Cat No. GTX100713

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

223 kDa. ( Note )

Positive Control

mouse muscle

Predict Reactivity

Human(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myosin Heavy Chain 7,Cmd1S,Cmh1,Mpd1,Myhcb,Spmd,Spmm,Myh7

Cellular Localization

Cytoplasm , myofibril

Background

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100713_40058_20160505_WB_M_muscle_19011506_580.jpg

GTX100713 WB Image

Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH7 antibody [N1], N-term (GTX100713) diluted at 1:2000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169