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MYH8 antibody

Anti-MYH8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32145
Anti-MYH8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32145
Anti-MYH8 antibody used in Western Blot (WB). GTX32145

Cat No. GTX32145

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($339)
See all MYH8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

223 kDa. ( Note )

Positive Control

HeLa Cell Lysate

Specificity/Sensitivity

MYH8 antibody is predicted to not cross-react with other members of the myosin heavy chain family.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MYH8 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH8.The immunogen is located within amino acids 60 - 110 of MYH8.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myosin heavy chain 8 , DA7 , MyHC-peri , MyHC-pn , gtMHC-F

Background

Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is predominantly expressed in fetal skeletal muscle. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in trismus-pseudocamptodactyly syndrome. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-MYH8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32145

GTX32145 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX32145 MYH8 antibody.
Working concentration : 20 μg/ml

Anti-MYH8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32145

GTX32145 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX32145 MYH8 antibody.
Working concentration : 5 μg/ml

Anti-MYH8 antibody used in Western Blot (WB). GTX32145

GTX32145 WB Image

WB analysis of HeLa cell lysate using GTX32145 MYH8 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339