MYH9 antibody [GT218]

Name: MYH9 antibody [GT218]
Brand: GeneTex
SKU: GTX633295
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-MYH9 antibody [GT218] used in Western Blot (WB). GTX633295

Anti-MYH9 antibody [GT218] used in IHC (Paraffin sections) (IHC-P). GTX633295

Anti-MYH9 antibody [GT218] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633295

Cat. No. GTX633295

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT218
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all MYH9 products

View product citations for antibody GTX633295 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
FACS	Assay dependent

Not tested in other applications.

Calculated MW

227 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Bovine, Dog, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888334

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin heavy chain 9 , BDPLT6 , DFNA17 , EPSTS , FTNS , MATINS , MHA , NMHC-II-A , NMMHC-IIA , NMMHCA

Background

This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq]

Database

Gene ID: 4627 MYH9
UniProt: P35579 MYH9

Research Area

DATA IMAGES

GTX633295 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [GT218] (GTX633295) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX633295 IHC-P Image

MYH9 antibody [GT218] detects MYH9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
MYH9 stained by MYH9 antibody [GT218] (GTX633295) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX633295 IHC-P Image

MYH9 antibody [GT218] detects MYH9 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MYH9 stained by MYH9 antibody [GT218] (GTX633295) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX633295 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [GT218] (GTX633295) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX633295 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [GT218] (GTX633295) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX633295 IHC-P Image

MYH9 antibody [GT218] detects MYH9 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MYH9 antibody [GT218] (GTX633295) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX633295 ICC/IF Image

MYH9 antibody [GT218] detects MYH9 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MYH9 stained by MYH9 antibody [GT218] (GTX633295) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Fekadu S et al. Cancer Sci 2021; (Epub) Gastric epithelial attachment of Helicobacter pylori induces EphA2 and NMHC-IIA receptors for Epstein-Barr virus.

Submit a Reference

REVIEW