APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , MYH9-transfected 293T , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
Predict Reactivity
Rat, Bovine, Dog, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MYH9. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888397
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
myosin heavy chain 9 , BDPLT6 , DFNA17 , EPSTS , FTNS , MATINS , MHA , NMHC-II-A , NMMHC-IIA , NMMHCA
Background
This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960](/templates/default/js/lightbox/images/loading.gif "Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960")
|
GTX633960 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [GT566] (GTX633960) diluted at 1:1000.
|
|
|
GTX633960 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [GT566] (GTX633960) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX633960 IHC-P Image
MYH9 antibody [GT566] detects MYH9 protein at cytoplasm in human mammary gland by immunohistochemical analysis.
Sample: Paraffin-embedded human mammary gland.
MYH9 antibody [GT566] (GTX633960) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX633960 IHC-P Image
MYH9 antibody [GT566] detects MYH9 protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
MYH9 antibody [GT566] (GTX633960) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX633960 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [GT566] (GTX633960) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for MYH9 antibody [GT566] (GTX633960). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MYH9 antibody [GT566] (GTX633960). Be the first to share your experience with this product.
![Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960](/upload/website/prouct_img/small/GTX633960/GTX633960_42744_20170209_WB_w_23061202_372.webp "Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960")
![Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960](/upload/website/prouct_img/small/GTX633960/GTX633960_42744_20170316_WB_B_w_23061202_128.webp "Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960")
![Anti-MYH9 antibody [GT566] used in IHC (Paraffin sections) (IHC-P). GTX633960](/upload/website/prouct_img/small/GTX633960/GTX633960_42744_20170426_IHC-P_1_w_23061202_328.webp "Anti-MYH9 antibody [GT566] used in IHC (Paraffin sections) (IHC-P). GTX633960")
![Anti-MYH9 antibody [GT566] used in IHC (Paraffin sections) (IHC-P). GTX633960](/upload/website/prouct_img/small/GTX633960/GTX633960_42744_20170426_IHC-P_2_w_23061202_559.webp "Anti-MYH9 antibody [GT566] used in IHC (Paraffin sections) (IHC-P). GTX633960")
![Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960](/upload/website/prouct_img/small/GTX633960/GTX633960_42744_20190118_WB_M_w_23061202_945.webp "Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960")
![Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960](/upload/website/prouct_img/normal/GTX633960/GTX633960_42744_20170209_WB_w_23061202_372.webp "Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960")
![Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960](/upload/website/prouct_img/normal/GTX633960/GTX633960_42744_20170316_WB_B_w_23061202_128.webp "Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960")
![Anti-MYH9 antibody [GT566] used in IHC (Paraffin sections) (IHC-P). GTX633960](/upload/website/prouct_img/normal/GTX633960/GTX633960_42744_20170426_IHC-P_1_w_23061202_328.webp "Anti-MYH9 antibody [GT566] used in IHC (Paraffin sections) (IHC-P). GTX633960")
![Anti-MYH9 antibody [GT566] used in IHC (Paraffin sections) (IHC-P). GTX633960](/upload/website/prouct_img/normal/GTX633960/GTX633960_42744_20170426_IHC-P_2_w_23061202_559.webp "Anti-MYH9 antibody [GT566] used in IHC (Paraffin sections) (IHC-P). GTX633960")
![Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960](/upload/website/prouct_img/normal/GTX633960/GTX633960_42744_20190118_WB_M_w_23061202_945.webp "Anti-MYH9 antibody [GT566] used in Western Blot (WB). GTX633960")