MYH9 antibody [N1-2], N-term

Cat. No. GTX113236

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all MYH9 products

View product citations for antibody GTX113236 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
IP	1:100-1:500

Not tested in other applications.

Calculated MW

227 kDa. ( Note )

Positive Control

Rat2 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Dog, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037478

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin heavy chain 9 , BDPLT6 , DFNA17 , EPSTS , FTNS , MATINS , MHA , NMHC-II-A , NMMHC-IIA , NMMHCA

Background

This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq]

Database

Gene ID: 4627 MYH9
UniProt: P35579 MYH9

Research Area

DATA IMAGES

GTX113236 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113236 IHC-P Image

MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113236 ICC/IF Image

MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MYH9 stained by MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX113236 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113236 IP Image

Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 μg of MYH9 antibody [N1-2], N-term (GTX113236).
Western blot analysis was performed using MYH9 antibody [N1-2], N-term (GTX113236).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX113236 WB Image

MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
MYH9 antibody [N1-2], N-term (GTX113236) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113236 IHC-P Image

MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113236 WB Image

MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
5% SDS-PAGE
MYH9 antibody [N1-2], N-term (GTX113236) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Yi Hu et al. Cancer Res 2021; 81 (3) : 713-723

Application : IHC-P

Reactivity : Human

Clare Rogerson et al. Cell Death Differ 2021; 28 (6) : 1849-1864 Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation.

Application : WB, ICC/IF

Reactivity : Human

Cheng HW et al. Life Sci Alliance 2019; 2 (1) Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration.

Application : WB

Reactivity : Human

Kwon HK et al. Biochim Biophys Acta 2018; (Epub) Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics.

Application : WB

Reactivity : Mouse

Sundvold H et al. Cell Cycle 2016; 15 (5) : 628-43 Arv1 promotes cell division by recruiting IQGAP1 and myosin to the cleavage furrow.

Application : WB

Reactivity : Human

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REVIEW

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Application Reference

Yi Hu et al. Cancer Res 2021; 81 (3):713-723

Application : IHC-P

Reactivity : Human

Clare Rogerson et al. Cell Death Differ 2021; 28 (6):1849-1864 Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation.

Application : WB, ICC/IF

Reactivity : Human

Cheng HW et al. Life Sci Alliance 2019; 2 (1) Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration.

Application : WB

Reactivity : Human

Application : WB

Reactivity : Mouse

Sundvold H et al. Cell Cycle 2016; 15 (5):628-43 Arv1 promotes cell division by recruiting IQGAP1 and myosin to the cleavage furrow.

Application : WB

Reactivity : Human

Li T et al. Elife 2016; 5 The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals.

Application : WB, IHC-Fr

Reactivity : Human

Sundvold H et al. Cell Cycle 2016; 15 (5) Arv1 promotes cell division by recruiting IQGAP1 and myosin to the cleavage furrow.

Application : WB, ICC/IF

Reactivity : Human

Law HT et al. J Proteome Res 2015; 14 (6):226634 Mass Spectrometry-Based Proteomics Identification of Enteropathogenic Escherichia coli Pedestal Constituents.

Application : ICC/IF

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

MYH9 antibody [N1-2], N-term

Cat. No. GTX113236

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX113236 WB Image

GTX113236 IHC-P Image

GTX113236 ICC/IF Image

GTX113236 WB Image

GTX113236 IP Image

GTX113236 WB Image

GTX113236 IHC-P Image

GTX113236 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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