GeneTex
United States (US)

MYH9 antibody [N1], N-term

GTX101751_40632_WB_1_19011506_208.jpg
GTX101751_40632_IHC_19011506_515.jpg
GTX101751_40632_WB_2_19011506_933.jpg
GTX101751_40632_20150508_IP_19011506_192.jpg

Cat No. GTX101751

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

227 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , NT2D1 , PC-3

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DFNA17 antibody, EPSTS antibody, FTNS antibody, MGC104539 antibody, MHA antibody, NMHC-II-A antibody, NMMHCA antibody, MYH9 antibody, myosin heavy chain 9 antibody, non-muscle myosin heavy chain IIa antibody, non-muscle myosin heavy chain A antibody, myosin-9 antibody, cellular myosin heavy chain, type A antibody, non-muscle myosin heavy polypeptide 9 antibody, myosin heavy chain, non-muscle IIa antibody, nonmuscle myosin heavy chain II-A antibody, myosin, heavy chain 9, non-muscle antibody

Background

This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101751_40632_WB_1_19011506_208.jpg

GTX101751 WB Image

MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis.
A. 30 µg U87-MG whole cell lysate/extract
B. 30 µg SK-N-SH whole cell lysate/extract
C. 30 µg IMR32 whole cell lysate/extract
D. 30 µg SK-N-AS whole cell lysate/extract
5 % SDS-PAGE
MYH9 antibody [N1], N-term (GTX101751) dilution: 1:1000

GTX101751_40632_IHC_19011506_515.jpg

GTX101751 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using MYH9(GTX101751) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101751_40632_WB_2_19011506_933.jpg

GTX101751 WB Image

MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis.
A. 30 µg NT2D1 whole cell lysate/extract
B. 30 µg PC-3 whole cell lysate/extract
5 % SDS-PAGE
MYH9 antibody [N1], N-term (GTX101751) dilution: 1:1000

GTX101751_40632_20150508_IP_19011506_192.jpg

GTX101751 IP Image

Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 μg of MYH9 antibody [N1], N-term (GTX101751).
Western blot analysis was performed using MYH9 antibody [N1], N-term (GTX101751).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169