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MYH9 antibody [N1], N-term

KO/KD Overexpression
Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751
Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751
Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751
Anti-MYH9 antibody [N1], N-term used in Immunoprecipitation (IP). GTX101751
Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751
Anti-MYH9 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101751
Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751
Anti-MYH9 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX101751

Cat. No. GTX101751

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MYH9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

227 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , NT2D1 , PC-3

Product Note

This antibody may cross react with MYH10, MYH14, or other family members.

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037477

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myosin heavy chain 9 , BDPLT6 , DFNA17 , EPSTS , FTNS , MATINS , MHA , NMHC-II-A , NMMHC-IIA , NMMHCA

Background

This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751

GTX101751 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term (GTX101751) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751

GTX101751 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term (GTX101751) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751

GTX101751 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term (GTX101751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MYH9 antibody [N1], N-term used in Immunoprecipitation (IP). GTX101751

GTX101751 IP Image

Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 μg of MYH9 antibody [N1], N-term (GTX101751).
Western blot analysis was performed using MYH9 antibody [N1], N-term (GTX101751).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751

GTX101751 WB Image

MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
5 % SDS-PAGE
MYH9 antibody [N1], N-term (GTX101751) dilution: 1:1000

Anti-MYH9 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101751

GTX101751 ICC/IF Image

MYH9 antibody [N1], N-term detects MYH9 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MYH9 stained by MYH9 antibody [N1], N-term (GTX101751) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-MYH9 antibody [N1], N-term used in Western Blot (WB). GTX101751

GTX101751 WB Image

MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis.
A. 30 μg NT2D1 whole cell lysate/extract
B. 30 μg PC-3 whole cell lysate/extract
5 % SDS-PAGE
MYH9 antibody [N1], N-term (GTX101751) dilution: 1:1000

Anti-MYH9 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX101751

GTX101751 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using MYH9(GTX101751) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Ruobo Zhou et al. Nat Commun 2022; 13 (1) : 3196 Proteomic and functional analyses of the periodic membrane skeleton in neurons.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169