MYH9 antibody [SF9]

Cat No. GTX33939

Host	Human
Clonality	Monoclonal
Clone Name	SF9
Isotype	IgG2
Application	WB, ICC/IF, ELISA, EM
Reactivity	Human, Mouse, Rat, Drosophila

See all MYH9 products

Datasheet

APPLICATION

Application Note

Immunocytochemistry: 1:1000, Western blot: 1:1000

Calculated MW

226 kDa. ( Note )

Specificity/Sensitivity

Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain).

PROPERTIES

Form

Liquid

Buffer

PBS containing 10% glycerol and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Full length myosin IIA from rat liver.

Purification

Protein A-affinity purified.

Purity

≧95% (SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Myosin, Heavy Chain 9, Non-Muscle-Like 1,Myh9L1

Background

cellular myosin; involved in cytokinesis [RGD, Feb 2006]

Database

Gene ID: 25745
UniProt: Q62812

Research Area

DATA IMAGES

GTX33939 Image

Schematic antibody structure.

GTX33939 ICC/IF Image

Human Myosin IIA (non-muscle) (heavy chain) is detected by immunocytochemistry using anti-myosin IIA (non-muscle) (rec.) (SF9). Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-Myosin IIA (non-muscle) (rec.) (SF9) (1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.