Human Myosin IIA (non-muscle) (heavy chain) is detected by immunocytochemistry using anti-myosin IIA (non-muscle) (rec.) (SF9). Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-Myosin IIA (non-muscle) (rec.) (SF9) (1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.