GeneTex
United States (US)

MYO5A antibody, Internal

GTX88050_141_IHC_18122102_960.jpg
GTX88050_2572_WB_18122102_177.jpg

Cat No. GTX88050

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC 1-3μg/ml

Note :

IHC
Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction.

Not tested in other applications.

Calculated MW

215 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88050-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ETKQLELDLN, from the internal region of the protein sequence according to NP_000250.3; NP_001135967.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myosin Va,Gs1,Myh12,Myo5,Myr12,Myo5A

Background

This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES
GTX88050_141_IHC_18122102_960.jpg

GTX88050 IHC Image

IHC analysis of mouse skeletal muscle (first panel, and in green in third panel) using GTX88050 MYO5A antibody, Internal. Alpha-bungaratoxin staining in middle panel and in red in third panel.
Dilution : 1µg/ml

GTX88050_2572_WB_18122102_177.jpg

GTX88050 WB Image

WB analysis of Jurkat lysate using GTX88050 MYO5A antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329