APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 1-3μg/ml |
|
Note :
IHC
Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88050-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).
Predict Reactivity
Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-ETKQLELDLN, from the internal region of the protein sequence according to NP_000250.3; NP_001135967.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
myosin VA , GS1 , MYH12 , MYO5 , MYR12
Background
This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]
Database
Research Area
DATA IMAGES
|
GTX88050 WB Image
WB analysis of Jurkat lysate using GTX88050 MYO5A antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX88050 IHC Image
IHC analysis of mouse skeletal muscle (first panel, and in green in third panel) using GTX88050 MYO5A antibody, Internal. Alpha-bungaratoxin staining in middle panel and in red in third panel.
Dilution : 1μg/ml
|
REFERENCE
There are currently no references for MYO5A antibody, Internal (GTX88050). Be the first to share your publications with this product.
REVIEW
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