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MYPT1 (phospho Ser668) antibody

Anti-MYPT1 (phospho Ser668) antibody used in Western Blot (WB). GTX00776

Cat No. GTX00776

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
200 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.09% (W/V) sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 641-674 amino acids from human MYPT1

Purification

Protein A purified followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein phosphatase 1 regulatory subunit 12A , M130 , MBS , MYPT1

Cellular Localization

Cytoplasm

Background

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-MYPT1 (phospho Ser668) antibody used in Western Blot (WB). GTX00776

GTX00776 WB Image

WB analysis of HeLa and Jurkat untrated or treated with Calyculin A (100nM) cell lysate using GTX00776 MYPT1 (phospho Ser668) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 329