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MYST1 antibody

Orthogonal
Anti-MYST1 antibody used in Western Blot (WB). GTX129380

Cat. No. GTX129380

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MYST1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , 293T NE , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MYST1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine acetyltransferase 8 , MOF , MYST1 , ZC2HC8 , hMOF

Cellular Localization

Nucleus

Background

The MYST family of histone acetyltransferases, which includes MYST1, is named for the founding members MOZ (MYST3; MIM 601408), yeast YBF2 and SAS2, and TIP60 (HTATIP; MIM 601409). All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein-protein interactions and targeting transcriptional regulators to chromatin (Neal et al., 2000 [PubMed 10786633]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MYST1 antibody used in Western Blot (WB). GTX129380

GTX129380 WB Image

Orthogonal

MYST1 antibody detects MYST1 protein by western blot analysis. 293T whole cell extracts and nuclear extracts (30 μg) and other whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with MYST1 antibody (GTX129380) diluted by 1:1000

REFERENCE

Application Reference

Collins HM et al. BMC Cancer 2013; 13 : 37 Differential effects of garcinol and curcumin on histone and p53 modifications in tumour cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169