GeneTex

  • Country / Location Selection

United States (US)

Macro H2A.1 antibody [GT1329]

Recombinant antibody
Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241
Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241
Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241
Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241
Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241
Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241
Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241
Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241

Cat. No. GTX03241

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1329

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all Macro H2A.1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human macroH2A.1 .

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family member Y , H2A.y , H2A/y , H2AF12M , MACROH2A1.1 , mH2A1 , macroH2A1.2

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241

GTX03241 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Macro H2A.1 antibody [GT1329] (GTX03241) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241

GTX03241 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Macro H2A.1 antibody [GT1329] (GTX03241) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241

GTX03241 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX03241 Macro H2A.1 antibody [GT1329].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241

GTX03241 IHC-P Image

IHC-P analysis of mouse lung tissue section using GTX03241 Macro H2A.1 antibody [GT1329].
Dilution : 1:100

Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241

GTX03241 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX03241 Macro H2A.1 antibody [GT1329].
Dilution : 1:100

Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241

GTX03241 ICC/IF Image

ICC/IF analysis of C6 cells using GTX03241 Macro H2A.1 antibody [GT1329].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241

GTX03241 IHC-P Image

IHC-P analysis of human liver cancer section using GTX03241 Macro H2A.1 antibody [GT1329].
Dilution : 1:100

Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241

GTX03241 WB Image

WB analysis of various samples using GTX03241 Macro H2A.1 antibody [GT1329].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

There are currently no references for Macro H2A.1 antibody [GT1329] (GTX03241). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Macro H2A.1 antibody [GT1329] (GTX03241). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 439