APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human macroH2A.1 .
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
H2A histone family member Y , H2A.y , H2A/y , H2AF12M , MACROH2A1.1 , mH2A1 , macroH2A1.2
Cellular Localization
Nucleus
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
Database
Research Area
DATA IMAGES
![Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241](/templates/default/js/lightbox/images/loading.gif "Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241")
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GTX03241 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Macro H2A.1 antibody [GT1329] (GTX03241) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX03241 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Macro H2A.1 antibody [GT1329] (GTX03241) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX03241 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX03241 Macro H2A.1 antibody [GT1329].
Blue : DAPI for nuclear staining
Dilution : 1:100
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GTX03241 IHC-P Image
IHC-P analysis of mouse lung tissue section using GTX03241 Macro H2A.1 antibody [GT1329].
Dilution : 1:100
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GTX03241 IHC-P Image
IHC-P analysis of rat brain tissue section using GTX03241 Macro H2A.1 antibody [GT1329].
Dilution : 1:100
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GTX03241 ICC/IF Image
ICC/IF analysis of C6 cells using GTX03241 Macro H2A.1 antibody [GT1329].
Blue : DAPI for nuclear staining
Dilution : 1:100
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GTX03241 IHC-P Image
IHC-P analysis of human liver cancer section using GTX03241 Macro H2A.1 antibody [GT1329].
Dilution : 1:100
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GTX03241 WB Image
WB analysis of various samples using GTX03241 Macro H2A.1 antibody [GT1329].
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
There are currently no references for Macro H2A.1 antibody [GT1329] (GTX03241). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Macro H2A.1 antibody [GT1329] (GTX03241). Be the first to share your experience with this product.
![Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241](/upload/website/prouct_img/small/GTX03241/GTX03241_4000001396_20210716_WB_2_w_23053123_861.webp "Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241](/upload/website/prouct_img/small/GTX03241/GTX03241_4000001396_20210716_WB_w_23053123_831.webp "Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241](/upload/website/prouct_img/small/GTX03241/GTX03241_20210615_ICCIF_60_w_23053123_961.webp "Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241](/upload/website/prouct_img/small/GTX03241/GTX03241_20210615_IHC-P_58_w_23053123_417.webp "Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241](/upload/website/prouct_img/small/GTX03241/GTX03241_20210615_IHC-P_56_w_23053123_294.webp "Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241](/upload/website/prouct_img/small/GTX03241/GTX03241_20210615_ICCIF_59_w_23053123_451.webp "Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241](/upload/website/prouct_img/small/GTX03241/GTX03241_20210615_IHC-P_57_w_23053123_308.webp "Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241](/upload/website/prouct_img/small/GTX03241/GTX03241_54_WB_w_23053123_801.webp "Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241](/upload/website/prouct_img/normal/GTX03241/GTX03241_4000001396_20210716_WB_2_w_23053123_861.webp "Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241](/upload/website/prouct_img/normal/GTX03241/GTX03241_4000001396_20210716_WB_w_23053123_831.webp "Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241](/upload/website/prouct_img/normal/GTX03241/GTX03241_20210615_ICCIF_60_w_23053123_961.webp "Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241](/upload/website/prouct_img/normal/GTX03241/GTX03241_20210615_IHC-P_58_w_23053123_417.webp "Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241](/upload/website/prouct_img/normal/GTX03241/GTX03241_20210615_IHC-P_56_w_23053123_294.webp "Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241](/upload/website/prouct_img/normal/GTX03241/GTX03241_20210615_ICCIF_59_w_23053123_451.webp "Anti-Macro H2A.1 antibody [GT1329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241](/upload/website/prouct_img/normal/GTX03241/GTX03241_20210615_IHC-P_57_w_23053123_308.webp "Anti-Macro H2A.1 antibody [GT1329] used in IHC (Paraffin sections) (IHC-P). GTX03241")
![Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241](/upload/website/prouct_img/normal/GTX03241/GTX03241_54_WB_w_23053123_801.webp "Anti-Macro H2A.1 antibody [GT1329] used in Western Blot (WB). GTX03241")