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Macro H2A.1 antibody [RM248]

Anti-Macro H2A.1 antibody [RM248] used in Western Blot (WB). GTX33622
Anti-Macro H2A.1 antibody [RM248] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33622

Cat No. GTX33622

Host Rabbit
Clonality Monoclonal
Clone Name RM248
Isotype IgG
Application WB, ICC/IF, ELISA, Multiplexing
Reactivity Human
Package
100 μg ($349)
APPLICATION

Application Note

Western Blot: 0.5 ug/mL – 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.2 ug/mL – 1 ug/mL.

Calculated MW

40 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of human Histone macroH2A1.

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H2A Histone Family Member Y , H2A.Y , H2A/Y , H2Af12M , Macroh2A1.1 , Mh2A1 , Macroh2A1.2 , H2Afy

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Macro H2A.1 antibody [RM248] used in Western Blot (WB). GTX33622

GTX33622 WB Image

Western Blot of acid extracts from K562 cells, using anti-Histone macroH2A1 Rabbit mAb RM248 at 1 ug/mL.

Anti-Macro H2A.1 antibody [RM248] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33622

GTX33622 ICC/IF Image

Immunocytochemistry of HeLa cells, using anti-Histone macroH2A1 Rabbit mAb RM248 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

REFERENCE
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