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Macro H2A.1 antibody [RM248]

Anti-Macro H2A.1 antibody [RM248] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33622
Anti-Macro H2A.1 antibody [RM248] used in Western Blot (WB). GTX33622

Cat No. GTX33622

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM248

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL - 2 μg/mL
ICC/IF 1 μg/mL - 2 μg/mL
ELISA 0.2 μg/mL - 1 μg/mL
Not tested in other applications.

Specificity/Sensitivity

This antibody reacts to the Core histone macro-H2A.1 and macro-H2A.2 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.09% sodium azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of human Histone macroH2A1.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IGL,IGL@,IGLC6,immunoglobulin lambda locus

Background

Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments and three C segments are known to be incapable of encoding a protein and are considered pseudogenes. The locus also includes several non-immunoglobulin genes, many of which are pseudogenes or are predicted by automated computational analysis or homology to other species. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Macro H2A.1 antibody [RM248] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33622

GTX33622 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33622 Macro H2A.1 antibody [RM248].
Red : Primary antibody
Green : Actin

Anti-Macro H2A.1 antibody [RM248] used in Western Blot (WB). GTX33622

GTX33622 WB Image

WB analysis of acid extracts from K562 cells using GTX33622 Macro H2A.1 antibody [RM248].
Dilution : 1μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 399