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Macro H2A.1 antibody

Anti-Macro H2A.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32709
Anti-Macro H2A.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32709
Anti-Macro H2A.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32709
Anti-Macro H2A.1 antibody used in Western Blot (WB). GTX32709
Anti-Macro H2A.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32709
Anti-Macro H2A.1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32709

Cat No. GTX32709

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human H2AFY (NP_613258.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H2A histone family member Y , H2A.y , H2A/y , H2AF12M , MACROH2A1.1 , mH2A1 , macroH2A1.2

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Macro H2A.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32709

GTX32709 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX32709 Macro H2A.1 antibody.
Dilution : 1:100

Anti-Macro H2A.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32709

GTX32709 IHC-P Image

IHC-P analysis of mouse lung cancer tissue using GTX32709 Macro H2A.1 antibody.
Dilution : 1:100

Anti-Macro H2A.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32709

GTX32709 IHC-P Image

IHC-P analysis of human kidney tissue using GTX32709 Macro H2A.1 antibody.
Dilution : 1:100

Anti-Macro H2A.1 antibody used in Western Blot (WB). GTX32709

GTX32709 WB Image

WB analysis of various samples using GTX32709 Macro H2A.1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Macro H2A.1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32709

GTX32709 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32709 Macro H2A.1 antibody.
Dilution : 1:100

Anti-Macro H2A.1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32709

GTX32709 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32709 Macro H2A.1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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