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Macrophage + Monocyte antibody [MAC387]

Cat. No. GTX76577

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MAC387

Isotype

 IgG1

Application

 IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Goat, Bovine, Cat, Dog, Guinea pig, Baboon, Cynomolgus monkey, Fallow deer, Horse, MarmosetPig, Mink, Pygmy hippopotamus, Rhesus Monkey, Squirrel monkey
Package
100 μg ($389)
See all Macrophage + Monocyte products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1/100-1/200
IHC-Fr 1/100-1/200
FACS 1/50-1/100

Note :

IHC-P
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

FACS
Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Product Note

The antigen recognized by clone MCA387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human monocytes.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377426

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]

Research Area

REFERENCE

Application Reference

MY Salas-Fajardo et al. Vet Res Commun 2023; Fatal toxoplasmosis in a captive squirrel monkey (Saimiri boliviensis) in Portugal
Application : IHC-P
Reactivity : Squirrel monkey
Passmore MR et al. Respir Res 2018; 19 (1) : 231 Inflammation and lung injury in an ovine model of fluid resuscitated endotoxemic shock.
Application : IHC-P
Reactivity : Human
Lagumersindez-Denis N et al. Acta Neuropathol 2017; (Epub) Differential contribution of immune effector mechanisms to cortical demyelination in multiple sclerosis.
Application : IHC-P
Reactivity : Mouse
Passmore M et al. Am J Physiol Lung Cell Mol Physiol 2016; : ajplung.00296.2016 Inflammation and Lung Injury in an Ovine Model of Extracorporeal Membrane Oxygenation Support.
Application : IHC-P

Clone Reference

Kramann N et al. Brain Pathol 2014; Increased Meningeal T and Plasma Cell Infiltration Is Associated with Early Subpial Cortical Demyelination in Common Marmosets with Experimental Autoimmune Encephalomyelitis.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 389