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Macrophage + Monocytes antibody [MAC387]

Cat No. GTX76577

Host

Mouse

Clonality

Monoclonal

Clone Name

MAC387

Isotype

IgG1

Application

IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse, Rat, Rabbit, Goat, Bovine, Cat, Dog, Guinea pig, Baboon, Cynomolgus monkey, Fallow deer, Horse, MarmosetPig, Mink, Pygmy hippopotamus, Rhesus Monkey
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1/100-1/200
IHC-Fr 1/100-1/200
FACS 1/50-1/100

Note :

IHC-P
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

FACS
Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Specificity/Sensitivity

The antigen recognized by clone MCA387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human monocytes.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]

Research Area

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319