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Malate Dehydrogenase antibody (HRP)

Anti-Malate Dehydrogenase antibody (HRP) used in Western Blot (WB). GTX40570

Cat. No. GTX40570

Reference 1

Host

 Sheep

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA, IHC

Reactivity

 Pig
Package
1 mg ($399)
See all MDH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
IP 1:100
ELISA 1:10000-1:50000
IHC 1:500-:2500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Malate Dehydrogenase collected from porcine heart

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_424147

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malate dehydrogenase 1 , MDH

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-Malate Dehydrogenase antibody (HRP) used in Western Blot (WB). GTX40570

GTX40570 WB Image

WB analysis of pig malate dehydrogenase protein using GTX40570 Malate Dehydrogenase antibody (HRP).
Loading : 50 ng
Dilution : 1:1000

REFERENCE

Application Reference

Bezawork-Geleta A et al. Sci Rep 2015; 5 : 17397 LON is the master protease that protects against protein aggregation in human mitochondria through direct degradation of misfolded proteins.
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REVIEW

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Package List Price ($)
$ 399