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Malate dehydrogenase protein, His tag (active)

Malate dehydrogenase protein, His tag (active). GTX66891-pro

Cat No. GTX66891-pro

Application

Functional Assay

Species

E. coli
Package
100 μg ($289)

APPLICATION

Application Note

Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that cleaves 1.0 μmole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH 8.0 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.0) with 1 mM DTT, 10% glycerol, 50 mM NaCl.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 336 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

malate dehydrogenase, NAD(P)-binding , ECK3225 , JW3205

Database

Research Area

DATA IMAGES

Malate dehydrogenase protein, His tag (active). GTX66891-pro

GTX66891-pro Image

3 μg of GTX66891-pro Malate dehydrogenase protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf