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Maltose binding protein antibody

Anti-Maltose binding protein antibody used in Immunoprecipitation (IP). GTX124267
Anti-Maltose binding protein antibody used in Western Blot (WB). GTX124267

Cat No. GTX124267

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP
Package
100 μl ($329),
25 μl ($169)
See all Maltose binding protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:50000
IP 1:100-1:500
Not tested in other applications.

Positive Control

E.coli induction with 1mM IPTG

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7), 1% BSA. 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Maltose binding protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MBP , Maltose-Binding Protein

Research Area

DATA IMAGES

Anti-Maltose binding protein antibody used in Immunoprecipitation (IP). GTX124267

GTX124267 IP Image

MBP antibody Immunoprecipitation of MBP tag protein. E. coli lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 0.5 ug of anti-MBP antibody (GTX124267). (A) Input, 0.2ug of E.coli lysates. The precipitated protein was detected by GTX124267 diluted at 1:2000. EasyBlot anti-Rabbit IgG Kit was used in Western blot.

Anti-Maltose binding protein antibody used in Western Blot (WB). GTX124267

GTX124267 WB Image

Sample (0.5ul of whole cell lysate)

A: E.coli non-induction

B: E.coli induction with 1mM IPTG

10% SDS PAGE

GTX124267 diluted at 1:50000

REFERENCE

Application Reference

Huang HY et al. Plant Methods 2019; 15 : 80 Heterologous overexpression, purification and functional analysis of plant cellulose synthase from green bamboo.
Application : WB
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169