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Mannose Receptor antibody [MR5D3]

Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264
Anti-Mannose Receptor antibody [MR5D3] used in Flow cytometry (FACS). GTX42264
Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264
Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264
Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264
Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264

Cat. No. GTX42264

Reference 9

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MR5D3

Isotype

 IgG2a

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Mouse, Rat
Package
100 μg ($399)
See all Mannose Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/10-1/20
IP Assay dependent

Note :

FACS
CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

165 kDa. ( Note )

Product Note

Clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Chimaeric CRD4-7-Fc protein.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11167654

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mannose receptor, C type 1 , AW259686 , CD206 , MR

Cellular Localization

Endosome membrane , Cell membrane

Database

Research Area

DATA IMAGES

Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264

GTX42264 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42264 Mannose Receptor antibody [MR5D3].

Anti-Mannose Receptor antibody [MR5D3] used in Flow cytometry (FACS). GTX42264

GTX42264 FACS Image

FACS analysis of J774 cells using GTX42264 Mannose Receptor antibody [MR5D3].

Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264

GTX42264 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42264 Mannose Receptor antibody [MR5D3].

Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264

GTX42264 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42264 Mannose Receptor antibody, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained in blue using DAPI.

Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264

GTX42264 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42264 Mannose Receptor antibody [MR5D3].

Anti-Mannose Receptor antibody [MR5D3] used in IHC (Frozen sections) (IHC-Fr). GTX42264

GTX42264 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42264 Mannose Receptor antibody, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained in blue using DAPI.

REFERENCE

Application Reference

Liu JR et al. Food Sci Nutr 2023; 11 (11) : 6974-6986 Polygonatum odoratum polysaccharide attenuates lipopolysaccharide-induced lung injury in mice by regulating gut microbiota.
Li J et al. Ann Transl Med 2021; 9 (20) : 1534 Lupeol reduces M1 macrophage polarization to attenuate immunologic dissonance and fatty acid deposition in rats with diet-induced metabolic syndrome.
Application : FACS
Reactivity : Rat

Clone Reference

Hsu AY et al. Front Cell Neurosci 2022; 16:810647 Role of Dectin-1 in peripheral nerve injury.
Bin-Bin Z et al. J Orthop Surg Res 2022; 17 (1):137 M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cells.
Da-Wa ZX et al. Biomed Res Int 2021; 2021:7218067 Exosomes Derived from M2 Macrophages Exert a Therapeutic Effect via Inhibition of the PI3K/AKT/mTOR Pathway in Rats with Knee Osteoarthritic.
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REVIEW

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Application Reference
Liu JR et al. Food Sci Nutr 2023; 11 (11):6974-6986 Polygonatum odoratum polysaccharide attenuates lipopolysaccharide-induced lung injury in mice by regulating gut microbiota.
Li J et al. Ann Transl Med 2021; 9 (20):1534 Lupeol reduces M1 macrophage polarization to attenuate immunologic dissonance and fatty acid deposition in rats with diet-induced metabolic syndrome.
Application : FACS
Reactivity : Rat
Hsieh CC et al. Food Chem Toxicol 2021; 147:111908 Seed peptide lunasin ameliorates obesity-induced inflammation and regulates immune responses in C57BL/6J mice fed high-fat diet.
Application : IHC-P
Reactivity : Mouse
Zhong Q et al. Prog Neuropsychopharmacol Biol Psychiatry 2019; 90:62-75 FCPR16, a novel phosphodiesterase 4 inhibitor, produces an antidepressant-like effect in mice exposed to chronic unpredictable mild stress.
Application : IHC-Fr
Reactivity : Mouse
Clone Reference
Hsu AY et al. Front Cell Neurosci 2022; 16:810647 Role of Dectin-1 in peripheral nerve injury.
Bin-Bin Z et al. J Orthop Surg Res 2022; 17 (1):137 M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cells.
Da-Wa ZX et al. Biomed Res Int 2021; 2021:7218067 Exosomes Derived from M2 Macrophages Exert a Therapeutic Effect via Inhibition of the PI3K/AKT/mTOR Pathway in Rats with Knee Osteoarthritic.
Roberto Motterlini et al. Redox Biol . 2019; 20:334–348 HYCO-3, a dual CO-releaser/Nrf2 activator, reduces tissue inflammation in mice challenged with lipopolysaccharide
Application : IHC-P
Reactivity : Mouse
Principi E et al. Int J Obes (Lond) 2018; (Epub) Anthropometric and glucometabolic changes in an aged mouse model of lipocalin-2 overexpression.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399