Mannose Receptor antibody [MR5D3]

Cat. No. GTX42264

Reference 8

Host	Rat
Clonality	Monoclonal
Clone Name	MR5D3
Isotype	IgG2a
Application	ICC/IF, IHC-P, IHC-Fr, FACS, IP
Reactivity	Mouse, Rat
Package	100 μg ($399)

See all Mannose Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent
FACS	1/10-1/20
IP	Assay dependent
Note : FACS CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl.

Not tested in other applications.

Calculated MW

165 kDa. ( Note )

Product Note

Clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Chimaeric CRD4-7-Fc protein.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11167654

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mannose receptor, C type 1 , AW259686 , CD206 , MR

Cellular Localization

Endosome membrane , Cell membrane

Database

Gene ID: 17533 Mrc1
UniProt: Q61830 Mrc1

Research Area

DATA IMAGES

GTX42264 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42264 Mannose Receptor antibody [MR5D3].

GTX42264 FACS Image

FACS analysis of J774 cells using GTX42264 Mannose Receptor antibody [MR5D3].

GTX42264 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42264 Mannose Receptor antibody [MR5D3].

GTX42264 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42264 Mannose Receptor antibody, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained in blue using DAPI.