*Optimal dilutions/concentrations should be determined by the researcher.
Antibody PLK-1 stained MARCO under non-reducing conditions.
Tissue sections can be- fixed in acetone or 2% paraformaldehyde.
Antibody PLK-1 immunoprecipitates MARCO as 60 and 50 kDa protein from lysates obtained from COS cells transfected with human MARCO.
Antibody PLK-1 blocks human alveolar macrophages binding to unopsonized particles.
Not tested in other applications.
This antibody stains alveolar macrophages by recognizing the extracellular domain of MARCO. The monoclonal antibody PLK-1 binds specifically to MARCO, and has been shown to partially block ligand binding.
0.2μm-filtered PBS, 0.1% BSA
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
100 μg/ml (Please refer to the vial label for the specific concentration.)
Human alveolar macrophages (recognizes domain V between residues 420 and 431)
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
MARCO,SCARA2,macrophage receptor with collagenous structure,Marco
The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. [provided by RefSeq, Jul 2008]