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Marco antibody [PLK-1]

Cat. No. GTX54470

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PLK-1

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, Neutralizing/Inhibition

Reactivity

 Human, Bovine
Package
50 μg ($399)
See all Marco products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 0.6 μg/ml
IHC-Fr 5μg/ml
FACS Assay dependent
IP Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

WB
Antibody PLK-1 stained MARCO under non-reducing conditions.

IHC-Fr
Tissue sections can be- fixed in acetone or 2% paraformaldehyde.

IP
Antibody PLK-1 immunoprecipitates MARCO as 60 and 50 kDa protein from lysates obtained from COS cells transfected with human MARCO.

Neutralizing/Inhibition
Antibody PLK-1 blocks human alveolar macrophages binding to unopsonized particles.
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Product Note

This antibody stains alveolar macrophages by recognizing the extracellular domain of MARCO. The monoclonal antibody PLK-1 binds specifically to MARCO, and has been shown to partially block ligand binding.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human alveolar macrophages (recognizes domain V between residues 420 and 431)

Purification

Purified IgG3

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MARCO,SCARA2,macrophage receptor with collagenous structure,Marco

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 399