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Maspin antibody [AT2N6]

Anti-Maspin antibody [AT2N6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50010
Anti-Maspin antibody [AT2N6] used in Western Blot (WB). GTX50010

Cat No. GTX50010

Host

Mouse

Clonality

Monoclonal

Clone Name

AT2N6

Isotype

IgG2b

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1000 ~ 2000. Recommended starting dilution is 1:1000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Maspin (1-375aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Serpin Family B Member 5 , Pi5 , Maspin , Serpinb5

Background

Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas.

Database

Research Area

DATA IMAGES

Anti-Maspin antibody [AT2N6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50010

GTX50010 ICC/IF Image

Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green).

Anti-Maspin antibody [AT2N6] used in Western Blot (WB). GTX50010

GTX50010 WB Image

Cell lysates of HeLa(20?g) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf