APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml for 30 minutes at RT |
| Assay dependent |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human Tryptase protein fragment (around aa 115-233) (Exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TPS1 , TPS2 , TPSAB1 , TPSB1 , tryptase alpha/beta 1 , Mast cell tryptase
Cellular Localization
Secreted
Background
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Mast cell tryptase antibody [TPSAB1/1961] used in Protein Array (Protein Array). GTX17823](/templates/default/js/lightbox/images/loading.gif "Anti-Mast cell tryptase antibody [TPSAB1/1961] used in Protein Array (Protein Array). GTX17823")
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GTX17823 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using Tryptase Mouse Monoclonal Antibody (TPSAB1/1961). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX17823 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX17823 Mast cell tryptase antibody [TPSAB1/1961].
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REFERENCE
There are currently no references for Mast cell tryptase antibody [TPSAB1/1961] (GTX17823). Be the first to share your publications with this product.
REVIEW
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![Anti-Mast cell tryptase antibody [TPSAB1/1961] used in Protein Array (Protein Array). GTX17823](/upload/website/prouct_img/small/GTX17823/GTX17823_20200115_Protein-Array_2109_w_23060620_419.webp "Anti-Mast cell tryptase antibody [TPSAB1/1961] used in Protein Array (Protein Array). GTX17823")
![Anti-Mast cell tryptase antibody [TPSAB1/1961] used in IHC (Paraffin sections) (IHC-P). GTX17823](/upload/website/prouct_img/small/GTX17823/GTX17823_20200115_IHC-P_1415_w_23060620_578.webp "Anti-Mast cell tryptase antibody [TPSAB1/1961] used in IHC (Paraffin sections) (IHC-P). GTX17823")
![Anti-Mast cell tryptase antibody [TPSAB1/1961] used in Protein Array (Protein Array). GTX17823](/upload/website/prouct_img/normal/GTX17823/GTX17823_20200115_Protein-Array_2109_w_23060620_419.webp "Anti-Mast cell tryptase antibody [TPSAB1/1961] used in Protein Array (Protein Array). GTX17823")
![Anti-Mast cell tryptase antibody [TPSAB1/1961] used in IHC (Paraffin sections) (IHC-P). GTX17823](/upload/website/prouct_img/normal/GTX17823/GTX17823_20200115_IHC-P_1415_w_23060620_578.webp "Anti-Mast cell tryptase antibody [TPSAB1/1961] used in IHC (Paraffin sections) (IHC-P). GTX17823")