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Mast cell tryptase antibody

Anti-Mast cell tryptase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32931
Anti-Mast cell tryptase antibody used in Western Blot (WB). GTX32931

Cat No. GTX32931

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-210 of human TPSAB1 (NP_003285.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tryptase alpha/beta 1 , TPS1 , TPS2 , TPSB1 , TPSB2 , Tryptase-2

Cellular Localization

Secreted

Background

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-Mast cell tryptase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32931

GTX32931 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32931 Mast cell tryptase antibody.
Blue : DAPI

Anti-Mast cell tryptase antibody used in Western Blot (WB). GTX32931

GTX32931 WB Image

WB analysis of various samples using GTX32931 Mast cell tryptase antibody.
Dilution : 1:200
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 329