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Melan A antibody [DT101 + BC199]

Cat. No. GTX20731

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DT101 + BC199

Isotype

 IgG

Application

 IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
500 μl ($399)
See all Melan A products

APPLICATION

Application Note

IHC:For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC. It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas.

Positive Control

Melanoma

Product Note

This antibody recognizes a protein of 18kDa, identified at Melan A (Melanoma Antigen Recognized by T- cells 1) or Melan-A. The MART-1 recognizes a subcellular fraction found in melanosomes. The antibody labels melanomas and tumors showing melanocytic differentiation. It does not mark neoplasms of epithelial origin, lymphomas or mesenchymal tumors. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Both HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Studies have shown that MART-1 is more sensitive than HMB-45 when labeling metastatic melanomas.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant dilute with PBS pH7.3, protein carrier

Preservative

Proprietary preservative , not Sodium azide or Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Endoplasmic reticulum and melanosomes.

Background

MART-1 (Melanoma Antigen Recognized by T cells) or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 399