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Melan A antibody [MLANA/1761R]

Anti-Melan A antibody [MLANA/1761R] used in IHC (Paraffin sections) (IHC-P). GTX34830
Anti-Melan A antibody [MLANA/1761R] used in IHC (Paraffin sections) (IHC-P). GTX34830

Cat No. GTX34830

Host Rabbit
Clonality Monoclonal
Clone Name MLANA/1761R
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
See all Melan A products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human full-length MLANA protein

Purification

Ab purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Melan-A,Mart-1,Mart1,Mlana

Database

Research Area

DATA IMAGES
Anti-Melan A antibody [MLANA/1761R] used in IHC (Paraffin sections) (IHC-P). GTX34830

GTX34830 IHC-P Image

Formalin-fixed, paraffin-embedded Human Skin stained with MART-1 Recombinant Rabbit Monoclonal Antibody (MLANA/1761R)
Anti-Melan A antibody [MLANA/1761R] used in IHC (Paraffin sections) (IHC-P). GTX34830

GTX34830 IHC-P Image

Formalin-fixed, paraffin-embedded Human Melanoma stained with MART-1 Recombinant Rabbit Monoclonal Antibody (MLANA/1761R)
Package List Price ($)
$ 379