GeneTex
United States (US)

Melan A antibody [SPM342]

Anti-Melan A antibody [SPM342] used in IHC (Paraffin sections) (IHC-P). GTX34825

Cat No. GTX34825

Host Mouse
Clonality Monoclonal
Clone Name SPM342
Isotype IgG2b
Application WB, ICC/IF, IHC-P
Reactivity Human, Horse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Recombinant hMART-1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Melan-A,Mart-1,Mart1,Mlana

Database

Research Area

DATA IMAGES
Anti-Melan A antibody [SPM342] used in IHC (Paraffin sections) (IHC-P). GTX34825

GTX34825 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with MART-1 Ab (SPM342).

Package List Price ($)
$ 329