GeneTex
United States (US)

Melanoma antibody [HMB45]

Melanoma-antibody-HMB45_IHC (Formalin-fixed paraffin-embedded sections)_GTX20787-1_18121411_322.jpg
Melanoma-antibody-HMB45-GTX20787-IHC-1_18121411_119.jpg

Cat No. GTX20787

Host Mouse
Clonality Monoclonal
Clone Name HMB45
Isotype IgG1
Application IHC-P, IHC-Fr, IHC
Reactivity Human
APPLICATION

Application Note

IHC: This antibody may be diluted to a titer of 1:10-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval may increase staining intensity when using formalin fixed paraffin embedded tissue.

Specificity/Sensitivity

This antibody reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. It reacts with junctional and blue nevus cells. Non-melanocytic cells are negative.
PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant with PBS, 1% BSA and 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.025 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Extract of pigmented melanoma metastases from lymph nodes.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cutaneous Malignant Melanoma/Dysplastic Nevus,Cmm1,Dns,Fammm,Mlm,Cmm

Cellular Localization

Cytoplasmic

Research Area

DATA IMAGES
Melanoma-antibody-HMB45_IHC (Formalin-fixed paraffin-embedded sections)_GTX20787-1_18121411_322.jpg

GTX20787 IHC-P Image

Formalin fixed paraffin embedded human melanoma stained with Melanoma antibody (GTX20787).

Melanoma-antibody-HMB45-GTX20787-IHC-1_18121411_119.jpg

GTX20787 IHC Image

IHC Image using GTX20787 - Detection of Melanoma by IHC in Human Melanoma

REFERENCE
Package List Price ($)
$ 319