APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89304-PEP).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CPIGENSPLLSGQQ, from the C Terminus of the protein sequence according to NP_008859.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
premelanosome protein , D12S53E , ME20 , ME20-M , ME20M , P1 , P100 , PMEL17 , SI , SIL , SILV , gp100
Cellular Localization
Golgi apparatus
Background
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]
Database
Research Area
DATA IMAGES
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GTX89304 ICC/IF Image
ICC/IF analysis of paraformaldehyde fixed A431 cells using GTX89304 Melanoma gp100 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml
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GTX89304 WB Image
WB analysis of mouse skin lysate (35μg protein in RIPA buffer) using GTX89304 Melanoma gp100 antibody, C-term.
Dilution : 1μg/ml
Dilution : 1μg/ml
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REFERENCE
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REVIEW
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