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Melanoma gp100 antibody [HMB45]

Anti-Melanoma gp100 antibody [HMB45] used in IHC (Paraffin sections) (IHC-P). GTX34833
Anti-Melanoma gp100 antibody [HMB45] used in IHC (Paraffin sections) (IHC-P). GTX34833

Cat No. GTX34833

Host Mouse
Clonality Monoclonal
Clone Name HMB45
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Specificity/Sensitivity

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Extract of pigmented melanoma metastases from lymph nodes

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Premelanosome Protein,D12S53E,Me20,Me20-M,Me20M,P1,P100,Pmel17,Si,Sil,Silv,Gp100,Pmel

Background

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES
Anti-Melanoma gp100 antibody [HMB45] used in IHC (Paraffin sections) (IHC-P). GTX34833

GTX34833 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 / Melanosome Monoclonal Antibody (HMB45).

Anti-Melanoma gp100 antibody [HMB45] used in IHC (Paraffin sections) (IHC-P). GTX34833

GTX34833 IHC-P Image

Formalin-fixed, paraffin-embedded human Testis stained with gp100 / Melanosome Monoclonal Antibody (HMB45).

Package List Price ($)
$ 329