PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Skin: A moderate to strong cytoplasmic PMEL immunostaining should be seen in all melanocytes of the skin.
Negative Control
Colon: PMEL immunostaining should be completely absent in all cells.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human SILV protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
premelanosome protein , D12S53E , ME20 , ME20-M , ME20M , P1 , P100 , PMEL17 , SI , SIL , SILV , gp100
Cellular Localization
Golgi apparatus , Melanosome
Background
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]
Database
Research Area
DATA IMAGES
![Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382](/templates/default/js/lightbox/images/loading.gif "Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382")
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GTX04382 IHC-P Image
IHC-P analysis of human skin malignant melanoma tissue section using GTX04382 Melanoma gp100 antibody [MSVA-617R] HistoMAX™.
Strong cytoplasmic Melanoma gp100 immunostaining in all cells of a malignant melanoma of the skin.
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GTX04382 IHC-P Image
IHC-P analysis of human rectum mucosa tissue section using GTX04382 Melanoma gp100 antibody [MSVA-617R] HistoMAX™.
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GTX04382 IHC-P Image
IHC-P analysis of human skin tissue section using GTX04382 Melanoma gp100 antibody [MSVA-617R] HistoMAX™.
Strong Melanoma gp100 immunostaining in melanocytes of the skin.
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REFERENCE
There are currently no references for Melanoma gp100 antibody [MSVA-617R] HistoMAX™ (GTX04382). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Melanoma gp100 antibody [MSVA-617R] HistoMAX™ (GTX04382). Be the first to share your experience with this product.
![Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382](/upload/website/prouct_img/small/GTX04382/GTX04382_20230728_IHC-P_99_23072722_436.webp "Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382")
![Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382](/upload/website/prouct_img/small/GTX04382/GTX04382_20230728_IHC-P_229_23072723_973.webp "Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382")
![Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382](/upload/website/prouct_img/small/GTX04382/GTX04382_20230728_IHC-P_359_23072723_664.webp "Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382")
![Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382](/upload/website/prouct_img/normal/GTX04382/GTX04382_20230728_IHC-P_99_23072722_436.webp "Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382")
![Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382](/upload/website/prouct_img/normal/GTX04382/GTX04382_20230728_IHC-P_229_23072723_973.webp "Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382")
![Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382](/upload/website/prouct_img/normal/GTX04382/GTX04382_20230728_IHC-P_359_23072723_664.webp "Anti-Melanoma gp100 antibody [MSVA-617R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04382")