APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml for 30 minutes at RT |
| Assay dependent |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
We do not recommend use of this product for Rat samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Membranes from a human melanoma metastasis
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
D12S53E , ME20 , ME20M , P1 , P100 , PMEL , PMEL17 , SI , SIL , SILV , gp100 , premelanosome protein , Pmel 17
Cellular Localization
Endoplasmic reticulum membrane,Golgi apparatus,Melanosome,Endosome,Multivesicular body,Secreted
Background
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]
Database
Research Area
DATA IMAGES
![Anti-Melanoma gp100 antibody [NKI-beteb] used in IHC (Paraffin sections) (IHC-P). GTX34835](/templates/default/js/lightbox/images/loading.gif "Anti-Melanoma gp100 antibody [NKI-beteb] used in IHC (Paraffin sections) (IHC-P). GTX34835")
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GTX34835 IHC-P Image
IHC-P analysis of human melanoma tissue using GTX34835 Melanoma gp100 antibody [NKI-beteb].
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GTX34835 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using PMEL/gp100 Mouse Monoclonal Antibody (NKI-beteb). Z-. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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REFERENCE
There are currently no references for Melanoma gp100 antibody [NKI-beteb] (GTX34835). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Melanoma gp100 antibody [NKI-beteb] (GTX34835). Be the first to share your experience with this product.
![Anti-Melanoma gp100 antibody [NKI-beteb] used in IHC (Paraffin sections) (IHC-P). GTX34835](/upload/website/prouct_img/small/GTX34835/GTX34835_20200115_IHC-P_741_w_23060801_804.webp "Anti-Melanoma gp100 antibody [NKI-beteb] used in IHC (Paraffin sections) (IHC-P). GTX34835")
![Anti-Melanoma gp100 antibody [NKI-beteb] used in Protein Array (Protein Array). GTX34835](/upload/website/prouct_img/small/GTX34835/GTX34835_20200115_Protein-Array_2076_w_23060801_179.webp "Anti-Melanoma gp100 antibody [NKI-beteb] used in Protein Array (Protein Array). GTX34835")
![Anti-Melanoma gp100 antibody [NKI-beteb] used in IHC (Paraffin sections) (IHC-P). GTX34835](/upload/website/prouct_img/normal/GTX34835/GTX34835_20200115_IHC-P_741_w_23060801_804.webp "Anti-Melanoma gp100 antibody [NKI-beteb] used in IHC (Paraffin sections) (IHC-P). GTX34835")
![Anti-Melanoma gp100 antibody [NKI-beteb] used in Protein Array (Protein Array). GTX34835](/upload/website/prouct_img/normal/GTX34835/GTX34835_20200115_Protein-Array_2076_w_23060801_179.webp "Anti-Melanoma gp100 antibody [NKI-beteb] used in Protein Array (Protein Array). GTX34835")