GeneTex
United States (US)

Melanoma gp100 antibody [PMEL/1825R]

Anti-Melanoma gp100 antibody [PMEL/1825R] used in IHC (Paraffin sections) (IHC-P). GTX34838

Cat No. GTX34838

Host Rabbit
Clonality Monoclonal
Clone Name PMEL/1825R
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Specificity/Sensitivity

Cytotoxic T lymphocytes (CTL s) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human SILV protein

Purification

recombinant MAb purified by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Premelanosome Protein,D12S53E,Me20,Me20-M,Me20M,P1,P100,Pmel17,Si,Sil,Silv,Gp100,Pmel

Background

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES
Anti-Melanoma gp100 antibody [PMEL/1825R] used in IHC (Paraffin sections) (IHC-P). GTX34838

GTX34838 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Recombinant Rabbit Monoclonal Antibody (PMEL/1825R).

Package List Price ($)
$ 379