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Melanoma gp100 antibody [PMEL/2037]

Anti-Melanoma gp100 antibody [PMEL/2037] used in IHC (Paraffin sections) (IHC-P). GTX17772
Anti-Melanoma gp100 antibody [PMEL/2037] used in IHC (Paraffin sections) (IHC-P). GTX17772
Anti-Melanoma gp100 antibody [PMEL/2037] used in Protein Array (Protein Array). GTX17772

Cat No. GTX17772

Host

Mouse

Clonality

Monoclonal

Clone Name

PMEL/2037

Isotype

IgG1

Application

IHC-P, ELISA, Protein Array

Reactivity

Human
Package
100 μg ($469)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1-2μg/ml for 30 minutes at RT
ELISA 2-4μg/ml (for coating)
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A recombinant fragment (around aa 376-502) of human SILV protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

D12S53E , ME20 , ME20M , P1 , P100 , PMEL , PMEL17 , SI , SIL , SILV , gp100 , premelanosome protein , Pmel 17

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus,Melanosome,Endosome,Multivesicular body,Secreted

Background

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-Melanoma gp100 antibody [PMEL/2037] used in IHC (Paraffin sections) (IHC-P). GTX17772

GTX17772 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX17772 Melanoma gp100 antibody [PMEL/2037].

Anti-Melanoma gp100 antibody [PMEL/2037] used in IHC (Paraffin sections) (IHC-P). GTX17772

GTX17772 IHC-P Image

IHC-P analysis of human skin tissue using GTX17772 Melanoma gp100 antibody [PMEL/2037].

Anti-Melanoma gp100 antibody [PMEL/2037] used in Protein Array (Protein Array). GTX17772

GTX17772 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using PMEL/gp100 Mouse Monoclonal Antibody (PMEL/2037). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469