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Melanoma gp100 antibody [SPM142]

Anti-Melanoma gp100 antibody [SPM142] used in IHC (Paraffin sections) (IHC-P). GTX34834
Anti-Melanoma gp100 antibody [SPM142] used in IHC (Paraffin sections) (IHC-P). GTX34834

Cat. No. GTX34834

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM142

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all Melanoma gp100 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Product Note

We do not recommend use of this product for Rat,Dog samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Extract of pigmented melanoma metastases from lymph nodes

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

D12S53E , ME20 , ME20M , P1 , P100 , PMEL , PMEL17 , SI , SIL , SILV , gp100 , premelanosome protein , Pmel 17

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus,Melanosome,Endosome,Multivesicular body,Secreted

Background

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-Melanoma gp100 antibody [SPM142] used in IHC (Paraffin sections) (IHC-P). GTX34834

GTX34834 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX34834 Melanoma gp100 antibody [SPM142].

Anti-Melanoma gp100 antibody [SPM142] used in IHC (Paraffin sections) (IHC-P). GTX34834

GTX34834 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX34834 Melanoma gp100 antibody [SPM142].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529