GeneTex
United States (US)

Melanoma gp100 antibody [SPM286]

Anti-Melanoma gp100 antibody [SPM286] used in IHC (Paraffin sections) (IHC-P). GTX34836

Cat No. GTX34836

Host Mouse
Clonality Monoclonal
Clone Name SPM286
Isotype IgG2b
Application ICC/IF, IHC-P
Reactivity Human, Horse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Specificity/Sensitivity

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Membranes from a human melanoma metastasis

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Premelanosome Protein,D12S53E,Me20,Me20-M,Me20M,P1,P100,Pmel17,Si,Sil,Silv,Gp100,Pmel

Background

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES
Anti-Melanoma gp100 antibody [SPM286] used in IHC (Paraffin sections) (IHC-P). GTX34836

GTX34836 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Monoclonal Antibody (SPM286).

Package List Price ($)
$ 329