GeneTex
United States (US)

Mena antibody

ENAH-antibody-GTX87912-WB-1_18122102_532.jpg
ENAH-antibody-GTX87912-IHC-1_18122102_334.jpg

Cat No. GTX87912

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Specificity/Sensitivity

ENAH Antibody detects endogenous levels of total ENAH protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Mena.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein enabled homolog

Cellular Localization

Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at s

Background

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
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Database

Research Area

DATA IMAGES
ENAH-antibody-GTX87912-WB-1_18122102_532.jpg

GTX87912 WB Image

Western blot analysis of extracts from HepG2 cells, using ENAH Antibody. The lane on the right is treated with the synthesized peptide.

ENAH-antibody-GTX87912-IHC-1_18122102_334.jpg

GTX87912 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ENAH Antibody. The picture on the right is treated with the synthesized peptide.