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Mer2 antibody

Anti-Mer2 antibody used in Western Blot (WB). GTX48729

Cat. No. GTX48729

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Saccharomyces cerevisiae
Package
50 μg ($399)
See all Mer2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IP 1:100
ELISA 1:5000-1:25000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Saccharomyces cerevisiae

Immunogen

Synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rec107p , MER2

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-Mer2 antibody used in Western Blot (WB). GTX48729

GTX48729 WB Image

Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399