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Mer2 antibody

Anti-Mer2 antibody used in Western Blot (WB). GTX48729

Cat No. GTX48729

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Saccharomyces cerevisiae
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:10000
IP 1:100
ELISA 1:5000 - 1:25000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Saccharomyces cerevisiae

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rec107p , MER2

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-Mer2 antibody used in Western Blot (WB). GTX48729

GTX48729 WB Image

Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution.

REFERENCE

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SDS
Sodium Azide.pdf