Mer2 (phospho Ser30) antibody

Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts.  Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis.  Proteins were obtained using TCA precipitation.  The primary antibody was used at a 1:7,500 dilution.  Secondary antibody was used at 1:5,000 dilution.  Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.

Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phos-phorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5,000 dilution.