APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500 - 1:2000 |
1:50 - 1:200 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human Merlin (NP_000259.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
neurofibromin 2 , ACN , BANF , SCH
Cellular Localization
Nucleus,Cytoplasm, cytoskeleton
Background
This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX35202 ICC/IF Image
ICC/IF analysis of C6 cells using GTX35202 Merlin antibody. Blue : DAPI Dilution : 1:100
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GTX35202 WB Image
WB analysis of various sample lysates using GTX35202 Merlin antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX35202 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX35202 Merlin antibody. Blue : DAPI Dilution : 1:100
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GTX35202 WB Image
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35202 Merlin antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX35202 ICC/IF Image
ICC/IF analysis of L929 cells using GTX35202 Merlin antibody. Blue : DAPI Dilution : 1:100
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GTX35202 IP Image
IP analysis of SKOV3 cell lysate using GTX35202 Merlin antibody. Antibody amount : 3μg / 200μg lysate Dilution : 1:1000
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REFERENCE
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REVIEW
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