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Merlin antibody

KO/KD
Anti-Merlin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35202
Anti-Merlin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35202
Anti-Merlin antibody used in Western Blot (WB). GTX35202
Anti-Merlin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35202
Anti-Merlin antibody used in Western Blot (WB). GTX35202
Anti-Merlin antibody used in Immunoprecipitation (IP). GTX35202

Cat. No. GTX35202

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Merlin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human Merlin (NP_000259.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofibromin 2 , ACN , BANF , SCH

Cellular Localization

Nucleus,Cytoplasm, cytoskeleton

Background

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Merlin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35202

GTX35202 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX35202 Merlin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Merlin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35202

GTX35202 ICC/IF Image

ICC/IF analysis of L929 cells using GTX35202 Merlin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Merlin antibody used in Western Blot (WB). GTX35202

GTX35202 WB Image

WB analysis of various sample lysates using GTX35202 Merlin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Merlin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35202

GTX35202 ICC/IF Image

ICC/IF analysis of C6 cells using GTX35202 Merlin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Merlin antibody used in Western Blot (WB). GTX35202

GTX35202 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35202 Merlin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Merlin antibody used in Immunoprecipitation (IP). GTX35202

GTX35202 IP Image

IP analysis of SKOV3 cell lysate using GTX35202 Merlin antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399